And supporters will be able to watch the game live and exclusive on the Official Newcastle United App.
Kick-off is at 14:00 (BST) and defender Jodie Bartle - currently recovering from an ankle injury - will join Matthew Raisbeck on commentary.
Becky Langley's Magpies kicked off the campaign with an encouraging 1-1 draw at promotion contenders London City Lionesses earlier this month.
Former England defender Demi Stokes - one of nine summer signings - is set to make her home bow against the Blades, while Elysia Boddy penned a new three-year contract last weekend, following head coach Langley in committing her long-term future to the club.
Fan favourites like Katie Barker, Kacie Elson and Jasmine McQuade also have a big part to play in black and white, and tickets for the game are on sale here, while season tickets are still available from book.newcastleunited.com.
But fans who can't make the match can watch for free - anywhere in the world - via the Official Newcastle United App.
Download the Official Newcastle United App on the App Store or Google Play.