The January transfer window closed on Monday night, meaning all Premier League clubs had to resubmit their squads to the league this week.
Kieran Trippier, Chris Wood, Bruno Guimarães and Dan Burn all joined the Magpies during the window, while Matt Targett arrived on loan for the remainder of the campaign.
Isaac Hayden suffered a long-term knee injury in December, so has not been named in the squad for the second part of the season, while defenders Ciaran Clark and Jamal Lewis also miss out.
Newcastle United's 25-man squad for the second half of the season is: 1 Martin Dúbravka 3 Paul Dummett 5 Fabian Schär 6 Jamaal Lascelles 7 Joelinton 8 Jonjo Shelvey 9 Callum Wilson 10 Allan Saint-Maximin 11 Matt Ritchie 13 Matt Targett 15 Kieran Trippier 17 Emil Krafth 18 Federico Fernández 19 Javier Manquillo 20 Chris Wood 21 Ryan Fraser 23 Jacob Murphy 24 Miguel Almirón 26 Karl Darlow 28 Joe Willock 29 Mark Gillespie 33 Dan Burn 34 Dwight Gayle 36 Sean Longstaff 39 Bruno Guimarães