The international and domestic transfer windows have now closed, meaning all Premier League clubs had to submit their final squads to the league on Tuesday.
Dwight Gayle and Matt Ritchie are both included for the Magpies, with the club hopeful that they will have recovered from their respective injuries before January, when clubs can resubmit their 25-man squads.
Missing out on the squad are Christian Atsu, Henri Saivet, Achraf Lazaar and Rolando Aarons.
Head coach Steve Bruce said: "Selecting the squad is always difficult because you know you have to leave people out. With all the players, they're professionals and they want to play week in, week out.
"With the players we've brought in, it makes it even tougher but it's a decision you have to make.
"Those who aren't involved will continue to train and keep themselves right and once we hit December and January, we hope we can get them fixed up with clubs."
Matty Longstaff is under the age of the age of 21 and so does not need to named in the 25-man squad.
Senior players Florian Lejeune, Yoshinori Muto and Freddie Woodman are out on loan for the season.
Newcastle United's 25-man squad for the first half of the season is: 1 Martin Dúbravka 2 Ciaran Clark 3 Paul Dummett 4 Matty Longstaff* 5 Fabian Schär 6 Jamaal Lascelles 7 Andy Carroll 8 Jonjo Shelvey 9 Joelinton 10 Allan Saint-Maximin 11 Matt Ritchie 12 Dwight Gayle 13 Callum Wilson 14 Isaac Hayden 15 Jamal Lewis 16 Jeff Hendrick 17 Emil Krafth 18 Federico Fernández 19 Javier Manquillo 21 Ryan Fraser 22 DeAndre Yedlin 23 Jacob Murphy 24 Miguel Almirón 26 Karl Darlow 29 Mark Gillespie 36 Sean Longstaff
* Under-21