The 49-year-old, who lost his grandfather to prostate cancer, will join Preston North End manager Simon Grayson and ex-England skipper Terry Butcher in the fifth annual Football to Amsterdam ride in conjunction with the EFL from 9th-11th June.
Grayson will ride in honour of his late friend Steve Garbett, who passed away from the disease in September 2014 and the duo will start at opposite ends of the country: Ferdinand pedalling to Amsterdam from the Queen Elizabeth Olympic Park's Lee Valley VeloPark, London, and Grayson joining the northern leg, which kicks off at Oakwell, home of Barnsley FC.
Starting out as a charity challenge with 35 riders in 2013, the Football to Amsterdam bike ride from Prostate Cancer UK and the EFL reached an incredible £1million cumulative fundraising landmark in June, as 350 riders rode 145 miles to stop prostate cancer being a killer.
Ferdinand - who scored 50 goals in just 84 appearances for Newcastle in the 1990s - said: "Football to Amsterdam has been an amazing success over the past four years and I'm really looking forward to saddling up for the first time to raise money for a charity close to my heart, Prostate Cancer UK.
"Prostate cancer affects 330,000 people in the UK, a staggering statistic. One in eight men in the UK will be affected by this disease in their lifetime. But as a black man, my risk is even greater. One in four will be affected, and if there is a history of the disease in the family - like mine - those odds are even shorter.
"I'll admit, prostate cancer wasn't on my radar a decade ago; I knew nothing about it at all. Now I do. I lost my grandfather to prostate cancer and it's also affected other members of my family too. I'll be 50 in December, and need to set an example to my family and my peers, so I go and see my doctor every year without fail."
Grayson added: "I am delighted to support Prostate Cancer UK, a charity which is very close to my heart, and I'm looking forward to joining the ride for a second time in honour of my late friend Steve Garbett.
"'Garby' was a really close pal, who followed my career as a player and latterly as a manager and was always there to offer me support and advice. He was there for the good and the bad before sadly passing away two years ago.
"His brave ten-year fight opened my eyes about the dangers of this disease, and I'm proud to wear my Prostate Cancer UK 'Man of Men' pin badge on the touchline every game to raise awareness. That man represents you, me and everyone affected by this disease.
"I was honoured to ride from London to Amsterdam with Steve's son, Dave, two years ago and we'll be putting out another strong team as we start from Oakwell this time round, aiming to take our fundraising up beyond £100,000."
James Beeby, Director of Fundraising at Prostate Cancer UK, said: "One man every hour dies from prostate cancer; it's the most common cancer in men. But we want to make prostate cancer a disease that the next generation of men do not fear. We thank Les, Simon, Terry and all the riders for joining the fight."
Ben Wright, Commercial Director of the EFL, said: "We are proud of our long term charity partnership with Prostate Cancer UK and, fresh from breaking through the £1million fundraising barrier this year, we can't wait to saddle up for the 2017 edition of Football to Amsterdam.
"The EFL football family will always be Men United, and our continued work with Prostate Cancer UK again proves that the beautiful game can make a massive difference."
For information about taking part in the 2017 ride go to prostatecanceruk.org/amsterdam or contact the cycling team by calling 020 8222 7158 or emailing [email protected].
