Kick-off is at 7.45pm (GMT). Ben Dawson's under-21 side are one of only three Academy teams to reach the last 16 after beating Doncaster Rovers (3-1), Notts County (2-0) and Grimsby Town (3-2) in the group stages before overcoming Macclesfield Town on penalties after a 1-1 draw in round two.
Jack Ross' Black Cats, meanwhile, drew 0-0 with Stoke City's under-21s in their first group game before beating Carlisle United 3-1 and Morecambe 1-0 to secure qualification from Group A. They then beat Notts County 2-0 in the second round.
The game will be an all-ticket fixture. There will be no ticket sales on the day of the game and cash turnstiles will not be open on the night.
Ticket prices have been set at £15 for adults and £5 for under-16s and over-65s. Tickets will go on sale to Sunderland supporters - who must have a valid customer number and purchasing history recorded on the club’s database in order to buy tickets for this game - on Friday morning.
News on Newcastle United's allocation, as well as on-sale dates, will be announced on nufc.co.uk later this week.