It will be the Magpies' first game in the competition, with the Czech side coming to Whitley Park for a 7pm (UK time) kick-off.
And commentator John Middleton will be joined on the mic by Newcastle United and England under-21 international midfielder Isaac Hayden.
Already this season, we've brought you live commentary of United's Premier League 2 games against Norwich City and Wolverhampton Wanderers, which have featured the likes of Jonjo Shelvey, Dwight Gayle, Rolando Aarons and Jack Colback.
It is free to sign up for NUFC TV via the club's official website.
Fans are advised to ensure they are logged into their NUFC TV accounts in good time ahead of kick-off. Coverage will start shortly before the 7pm kick-off.
There will also be exclusive post-match reaction from Beardsley and his team after the final whistle, plus extended highlights on Wednesday.