The young Magpies, who last won the competition in 2018, have been handed a home tie against the Aristocrats - who compete in the Northern League Division Two.
United’s second-string reached the final in 2022 before suffering a narrow 3-2 defeat against Blyth Spartans at St. James’ Park.
Current holders, Morpeth Town, and Blyth Spartans have both received byes to the quarter-final stages.
A date and kick-off time will be announced on nufc.co.uk in due course.
Northumberland Senior Cup first round draw:
Newcastle Benfield vs Heaton Stannington
Ashington vs Newcastle University
Newcastle United U21s vs Newcastle Blue Star
Bedlington Terriers vs Prudhoe YC Seniors
Whitley Bay vs Blyth Town
North Shields vs West Allotment Celtic