Mitrović, who has been on loan at Fulham since January, is included in Mladen Krstajić’s initial 27-man squad for the finals, which is set to be trimmed to 23 before the tournament gets under way in June.
The 23-year-old helped his country reach the World Cup by scoring seven goals in qualifying, while he also played his part in helping the Cottagers make it to Saturday’s Championship play-off final, netting 12 league goals in 17 outings.
Serbia will face Brazil, Costa Rica and Switzerland in Group E at their first World Cup since 2010.